Journal: Bioactive Materials

Article Title: Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications

doi: 10.1016/j.bioactmat.2025.11.012

Figure Lengend Snippet: Evaluation of biocompatibility, adhesion and migration ability of both BMSCs and HUVECs on CoCrMo-Mg scaffold. (A) Biocompatibility of BMSCs: (a) CCK-8 assay; (b) Live/dead staining of BMSCs at day 3; (c) Quantitative statistics of cell proliferation. (B) Biocompatibility of HUVECs: (a) CCK-8 assay; (b) Live/dead staining of HUVECs at day 3; (c) Quantitative statistics of cell proliferation. (C) Cell adhesion of BMSCs: (a) FE-SEM and EDS images of BMSCs co-cultured with CoCrMo and CoCrMo-Mg scaffolds for 48 h; (b) Quantitative statistics of cell length; (c) Quantitative statistics of filopodia per cell. (D) Cell adhesion of HUVECs: (a) FE-SEM and EDS images of HUVECs; (b) Quantitative statistics of cell length; (c) Quantitative statistics of filopodia per cell. (E) The process of Transwell assay in BMSCs and HUVECs cultured with CoCrMo and CoCrMo-Mg scaffolds. (F) Crystalline violet staining of migrated cells. (G) Migration counts of BMSCs and HUVECs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs), and RAW264.7 murine macrophage cell line (RAW264.7) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Migration, CCK-8 Assay, Staining, Cell Culture, Transwell Assay

Journal: Bioactive Materials

Article Title: Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications

doi: 10.1016/j.bioactmat.2025.11.012

Figure Lengend Snippet: Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and HIF-1α in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs), and RAW264.7 murine macrophage cell line (RAW264.7) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, ALP Assay

Journal: Aging Cell

Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function

doi: 10.1111/acel.70400

Figure Lengend Snippet: PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere.

Techniques: Transformation Assay, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Cell Culture, MTT Assay, Western Blot, Expressing, Cell Cycle Assay